anti cox1 polyclonal antibody  (Cell Signaling Technology Inc)

Journal: Nature Communications

Article Title: Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signaling

doi: 10.1038/s41467-025-63892-3

Figure Lengend Snippet: Effect of supplementation with 10-formyl-THF or formate on cisplatin-resistant cell survival and apoptotic cell death in cells with DLAT knockdown. Cells cultured under sublethal doses of cisplatin were treated with 10-formyl-THF ( a ) or formate ( b ) at indicated concentrations for 48 h. Apoptosis (top) and cell viability (bottom) were determined. 10-formyl-THF rescues cisplatin-resistant cell growth ( c ) and MT-CO2 expression ( d ) in cells lacking K44 MTHFD2 acetylation. Cisplatin-treated cells expressing K44R MTHFD2 were subjected to 10 μM 10-formyl-THF for 24 h followed by annexin V staining, cell viability assay, and assessment of MT-CO1, MT-CO2, and COXIV levels by immunoblotting and quantitative RT-PCR. Cells expressing the acetyl-mimetic mutant form of MTHFD2 K44Q were included for comparison. Effect of MT-CO2 overexpression on cisplatin-resistant cell growth in cells lacking K44 MTHFD2 acetylation ( e ) or DLAT ( f ) by K44R MTHFD2 expression or DLAT knockdown, respectively. Data are mean ± SD from 4 independent biological replicates for ( d ) and 3 for ( a – c , e , f ). P values were determined by one-way ANOVA. Source data are provided as a Source Data file.

Article Snippet: Antibodies against MTHFD2 (41377/D8W9U), myc-Tag (2278/71D10), phospho-Histone gamma H2AX S139 (9718/20E3), phospho-53BP1 S1778 (2675), COX1/MT-CO1 (62101), COX2/MT-CO2 (31219), COX IV (4850/3E11), acetyl-lysine (9441), Bcl-xL (2762), Bcl2 (15071), Mcl-1 (39224/D5V5L), Bad (9268/11E3), Bim (2933/C34C5), PARP (9542), Histone H3 (4499/D1H2), LC3A/B-I/II (4108), and p62 (5114) were purchased from Cell Signaling Technology.

Techniques: Knockdown, Cell Culture, Expressing, Staining, Viability Assay, Western Blot, Quantitative RT-PCR, Mutagenesis, Comparison, Over Expression

Journal: iScience

Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB

doi: 10.1016/j.isci.2024.108959

Figure Lengend Snippet: Mitochondrial network show signs of disorganization in striatal (upper panels) and cortical (lower panels) regions of NAGLU −/− mice Forty μm-thick coronal brain sections of 8-month-old wild type (WT) and NAGLU −/− mice were stained with the mitochondrial marker mitochondrially encoded cytochrome c oxidase I (MT-CO1, cytochrome c oxidase subunit 1 COX1). Nuclei were stained with Hoechst.

Article Snippet: rabbit anti-COX1/MT-CO1 antibody , Cell Signaling Technology , Cat# 62101.

Techniques: Staining, Marker

Journal: iScience

Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB

doi: 10.1016/j.isci.2024.108959

Figure Lengend Snippet:

Article Snippet: rabbit anti-COX1/MT-CO1 antibody , Cell Signaling Technology , Cat# 62101.

Techniques: Recombinant, Modification, Protease Inhibitor, Expressing, Clone Assay, Control, Generated, Knock-Out, Software